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591 to 600 of 842 Results

1_Sequence_alignment_of_DesK.tab Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases Tabular Data - 229.8 KB - 224 Variables, 306 Observations - UNF:6:JChUDRZ6/doYy9jW2t+zgQ== Homologs of DesK in different organisms. Sequence alignment get from a BLASTP using DesKC as query and excluding other Bacilus from the data base by CLUSTALW
2_DesK_wt_and_variant-activities_Bgal.tab Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases Tabular Data - 458 B - 10 Variables, 10 Observations - UNF:6:+4DfY4QqrGJfiBAbXHTnww== Signal translation in DesK and its variants. Bacillus subtilis ∆desk cells expressing either DesK WT and its variants H335A, H335Y, H188E, H188Q, H335Q or H188Q/H335Q were grown at 37 °C to an OD550 of 0.3, and then divided in two flasks. One was maintained at 37 °C (red bars), a...
3_Western_blot-DesK_wt_and_variant.jpeg Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases JPEG Image - 36.0 KB - MD5: 7241e1860d16485d148aecf1c85b091b Expression and membrane integration levels of DesK variants. Western blots of the membrane fraction of Bacillus cells expressing each DesK variant (DesK WT, H335A, H335Y, H335Q, H188Q/H335Q, H188E and H188Q).
4_Autophosphorylation_curve_DesK_wt_and_variant.tab Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases Tabular Data - 435 B - 3 Variables, 24 Observations - UNF:6:AChpgPX6NtVSFF17J9LNVg== Phosphorylation kinetics of DesKC WT and H335Q. Eight samples taken in the initial 30 seconds were analyzed with radioactive ATP
5_EnvZ_wt_and_variant_activities_Bgal.tab Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases Tabular Data - 3.7 KB - 24 Variables, 27 Observations - UNF:6:ZPUlJAEn/sRUWeYCsfZZZQ== β-galactosidase activity from a PompC-lacZ reporter fusion was measured at low osmolarity (Sucrose 0%) or high osmolarity (Sucrose 15%)
6_DESK_HIS_protein model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 12.7 MB - MD5: a640b7c9c7edebabcbdc6af3497c9dfb DesK structure in the phosphotransfer state (PDB 7JJI), where the H188E mutation was reverted to its WT.
7_DESK_GLU_protein model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 14.0 MB - MD5: 26e5011338644080dc253017b9d892e2 DesK structure in the phosphotransfer state (PDB 7JJI), where the H188E mutation was modified to H188Q.
8_DESK_DESR_WT_complex model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 23.0 MB - MD5: 52c3017fbd2667898741e8de386229f6 Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server.
9_DESK_DESR_H335Y_complex model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 27.1 MB - MD5: 882c896df053c365b150957752e0cb99 Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server. The DesK His188 was mutated to tyrosine in silico
01_Transcription_levels_of_some_genes_involved_in_starch_metabolism_in_ChlreSEX4_overexpressing_algal_lines.tab Apr 24, 2024 - Transcription levels of some genes involved in starch metabolism in ChlreSEX4 overexpressing algal lines Tabular Data - 18.8 KB - 18 Variables, 103 Observations - UNF:6:bGlHIV4hObAsW7mDFL7Eiw== The file displays tables with the relative expression for each studied gene in different algal lines (WT, 13, 24, and 48), with each gene shown in different colors. The column "SAMPLE NAME" indicates which algal line is being analyzed. The column "TARGET NAME" indicates which gen...

1_Sequence_alignment_of_DesK.tab

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

Tabular Data - 229.8 KB - 224 Variables, 306 Observations -

Homologs of DesK in different organisms. Sequence alignment get from a BLASTP using DesKC as query and excluding other Bacilus from the data base by CLUSTALW

2_DesK_wt_and_variant-activities_Bgal.tab

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

Tabular Data - 458 B - 10 Variables, 10 Observations -

Signal translation in DesK and its variants. Bacillus subtilis ∆desk cells expressing either DesK WT and its variants H335A, H335Y, H188E, H188Q, H335Q or H188Q/H335Q were grown at 37 °C to an OD550 of 0.3, and then divided in two flasks. One was maintained at 37 °C (red bars), a...

3_Western_blot-DesK_wt_and_variant.jpeg

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

JPEG Image - 36.0 KB -

Expression and membrane integration levels of DesK variants. Western blots of the membrane fraction of Bacillus cells expressing each DesK variant (DesK WT, H335A, H335Y, H335Q, H188Q/H335Q, H188E and H188Q).

4_Autophosphorylation_curve_DesK_wt_and_variant.tab

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

Tabular Data - 435 B - 3 Variables, 24 Observations -

Phosphorylation kinetics of DesKC WT and H335Q. Eight samples taken in the initial 30 seconds were analyzed with radioactive ATP

5_EnvZ_wt_and_variant_activities_Bgal.tab

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

Tabular Data - 3.7 KB - 24 Variables, 27 Observations -

β-galactosidase activity from a PompC-lacZ reporter fusion was measured at low osmolarity (Sucrose 0%) or high osmolarity (Sucrose 15%)

6_DESK_HIS_protein model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 12.7 MB -

DesK structure in the phosphotransfer state (PDB 7JJI), where the H188E mutation was reverted to its WT.

7_DESK_GLU_protein model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 14.0 MB -

DesK structure in the phosphotransfer state (PDB 7JJI), where the H188E mutation was modified to H188Q.

8_DESK_DESR_WT_complex model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 23.0 MB -

Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server.

9_DESK_DESR_H335Y_complex model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 27.1 MB -

Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server. The DesK His188 was mutated to tyrosine in silico

01_Transcription_levels_of_some_genes_involved_in_starch_metabolism_in_ChlreSEX4_overexpressing_algal_lines.tab

Apr 24, 2024 - Transcription levels of some genes involved in starch metabolism in ChlreSEX4 overexpressing algal lines

Tabular Data - 18.8 KB - 18 Variables, 103 Observations -

The file displays tables with the relative expression for each studied gene in different algal lines (WT, 13, 24, and 48), with each gene shown in different colors. The column "SAMPLE NAME" indicates which algal line is being analyzed. The column "TARGET NAME" indicates which gen...

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