21 to 30 of 590 Results
Oct 23, 2024 - Facultad de Ciencias Bioquímicas y Farmacéuticas
Cricco, Julia Alejandra; Mediavilla, María Gabriela; Merli, Marcelo Luciano; Cobine, Paul Antony; Zhu, Xinyu, 2024, "Raw data about control of copper ion homeostasis in Trypanosoma cruzi", https://doi.org/10.57715/UNR/5XIFXN, RDA UNR, V1, UNF:6:9KTk//YxVvcbWQ++3f26FA== [fileUNF]
Introduction: Trypanosoma cruzi, the parasite that causes Chagas disease, requires of essential metabolites and cofactors to guarantee its survival. This parasite must supply its quota of metal ions such as copper (Cu), iron (Fe), calcium (Ca), etc. T. cruzi depends on a mitochon... |
Tabular Data - 200 B - 11 Variables, 7 Observations - UNF:6:Agi6/A8qejmx4Y1AXKjJ3A==
2021-12-23_27_Viability test_BCS
Vero cells were plated on 96 multi-well plate in DMEM-2 % FBS (4 x 103 cells per well) and incubated for 24 hours. Then, the cells were washed with PBS and incubated with 200 μL of DMEM-2 % FBS supplemented with 50-500 μM BCS. No addition was made... |
Tabular Data - 224 B - 14 Variables, 3 Observations - UNF:6:mhN7/VVzsm0oHy7eZHOKFw==
2021-12-30al 1-3_Viability test_Cu and NeoCup
Vero cells were plated on 96 multi-well plate in DMEM-2 % FBS (4 x 103 cells per well) and incubated for 24 hours. Then, the cells were washed with PBS and incubated with 200 μL of DMEM-2 % FBS supplemented with 50-500 μM Cu and 0.01-... |
Tabular Data - 48.6 KB - 48 Variables, 508 Observations - UNF:6:HYMizpe/IPodDRedM+aRNw==
Infection assay:
Vero cells (ATCC CCL-81) were seeded on 12 mm round coverslips in 24-well culture plates, at a density of 1.58 x 103 cells/cm2, and incubated in DMEM- 2 % FBS. After 24 h, they were infected with cell-derived trypomastigotes (MOI of 10) for 16 h in DMEM-2 % FBS s... |
Tabular Data - 1.5 KB - 18 Variables, 20 Observations - UNF:6:86qRTT5R5XnbDnqQMQnwkQ==
Growth curves_ General Method
The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p... |
Tabular Data - 1.8 KB - 20 Variables, 19 Observations - UNF:6:NC7+EC5F5tgnRBlsr7YEVw==
Growth curves_ General Method
The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p... |
Tabular Data - 1.3 KB - 4 Variables, 63 Observations - UNF:6:NA2hRqSDBRp29MQtOjThIA==
Quantification of metacyclogenesis:
Epimastigotes were maintained in mid-log phase by dilutions every 2-3 days in LIT-10 % FBS 5 μM hemin medium, at least for 10 days. Then, the cells were washed with PBS, diluted at a concentration of 5 x 106/mL and incubated in LIT-10 % FBS 5 μ... |
Tabular Data - 2.3 KB - 20 Variables, 27 Observations - UNF:6:thArFcR7AcyCuj8Nu1eRsA==
Growth curves_ General Method
The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p... |
Tabular Data - 509 B - 3 Variables, 27 Observations - UNF:6:B+hxSkK8spZK+CkDCydxFA==
2022-09-30_Oxygen consumption with Cu_BCS_NeoCup_day3
Oxygen consumption was measured in epimastigotes grown in LIT-10 % FBS-5 μM hemin medium plus copper (Cu), BCS, NeoCup or no addition (control), for 3 days. Concentration in μM.
The oxygen consumption rates from epimastigotes... |
Tabular Data - 124 B - 3 Variables, 5 Observations - UNF:6:WrzC/jcJWRlO3nSkM+QT3Q==
2022-10-17_Oxygen consumption with BCS_day 5
Oxygen consumption was measured in epimastigotes grown in LIT-10 % FBS-5 μM hemin medium plus BCS or no addition (control), for 5 days. Concentration in μM.
The oxygen consumption rates from epimastigotes were quantified from the line... |
