11 to 20 of 568 Results
Tabular Data - 48.6 KB - 48 Variables, 508 Observations - UNF:6:HYMizpe/IPodDRedM+aRNw==
Infection assay:
Vero cells (ATCC CCL-81) were seeded on 12 mm round coverslips in 24-well culture plates, at a density of 1.58 x 103 cells/cm2, and incubated in DMEM- 2 % FBS. After 24 h, they were infected with cell-derived trypomastigotes (MOI of 10) for 16 h in DMEM-2 % FBS s... |
Tabular Data - 1.5 KB - 18 Variables, 20 Observations - UNF:6:86qRTT5R5XnbDnqQMQnwkQ==
Growth curves_ General Method
The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p... |
Tabular Data - 1.8 KB - 20 Variables, 19 Observations - UNF:6:NC7+EC5F5tgnRBlsr7YEVw==
Growth curves_ General Method
The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p... |
Tabular Data - 1.3 KB - 4 Variables, 63 Observations - UNF:6:NA2hRqSDBRp29MQtOjThIA==
Quantification of metacyclogenesis:
Epimastigotes were maintained in mid-log phase by dilutions every 2-3 days in LIT-10 % FBS 5 μM hemin medium, at least for 10 days. Then, the cells were washed with PBS, diluted at a concentration of 5 x 106/mL and incubated in LIT-10 % FBS 5 μ... |
Tabular Data - 2.3 KB - 20 Variables, 27 Observations - UNF:6:thArFcR7AcyCuj8Nu1eRsA==
Growth curves_ General Method
The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p... |
Tabular Data - 509 B - 3 Variables, 27 Observations - UNF:6:B+hxSkK8spZK+CkDCydxFA==
2022-09-30_Oxygen consumption with Cu_BCS_NeoCup_day3
Oxygen consumption was measured in epimastigotes grown in LIT-10 % FBS-5 μM hemin medium plus copper (Cu), BCS, NeoCup or no addition (control), for 3 days. Concentration in μM.
The oxygen consumption rates from epimastigotes... |
Tabular Data - 124 B - 3 Variables, 5 Observations - UNF:6:WrzC/jcJWRlO3nSkM+QT3Q==
2022-10-17_Oxygen consumption with BCS_day 5
Oxygen consumption was measured in epimastigotes grown in LIT-10 % FBS-5 μM hemin medium plus BCS or no addition (control), for 5 days. Concentration in μM.
The oxygen consumption rates from epimastigotes were quantified from the line... |
Compressed Archive - 229.4 KB -
MD5: bd336bb7e278bb9a0bee9f711c94b670
The file “2022-11-08_Intracelular heme/2022-11-08_Samples.xlsx” shows the sample list for Heme content determination. The spectrums are recorded in separate files named as "PVC WAVE-SCAN 2022-11-08 ..." (22 files). Method: Intracellular heme content determined by pyridine method... |
Tabular Data - 94 B - 14 Variables, 3 Observations - UNF:6:UU9CR9M0aw9FG4gnMhbaZQ==
Superoxide anion production in epimastigotes
Superoxide anion production was quantified as it was previously described (https://doi.org/10.1016/j.cryobiol.2013.05.005) with some modifications. Epimastigotes were maintained for 10 days in mid-log growth phase by periodic dilutions... |
Tabular Data - 88.0 KB - 21 Variables, 578 Observations - UNF:6:ZEtNF1KCjgywO7+wvOVbUA==
ICP-OES
Cu, Fe, Ca, K, Mg, Mn, Na, P, S, and Zn contents were determined by ICP-OES as described in (https://doi.org/10.7554/eLife.64690). Briefly, epimastigotes were maintained for 6 days in LIT-10 % FBS-5 μM hemin medium plus different concentrations of copper or copper chelat... |
