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11 to 20 of 797 Results

2022-01-17_Effect of Cu and chelators in the infection_3rd.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 48.6 KB - 48 Variables, 508 Observations - UNF:6:HYMizpe/IPodDRedM+aRNw== Infection assay: Vero cells (ATCC CCL-81) were seeded on 12 mm round coverslips in 24-well culture plates, at a density of 1.58 x 103 cells/cm2, and incubated in DMEM- 2 % FBS. After 24 h, they were infected with cell-derived trypomastigotes (MOI of 10) for 16 h in DMEM-2 % FBS s...
2022-02-02_Growth curves 1_ Starved cells plus Cu 50 a 250.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 1.5 KB - 18 Variables, 20 Observations - UNF:6:86qRTT5R5XnbDnqQMQnwkQ== Growth curves_ General Method The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p...
2022-02-11_Growth curves 2_Copper trearment.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 1.8 KB - 20 Variables, 19 Observations - UNF:6:NC7+EC5F5tgnRBlsr7YEVw== Growth curves_ General Method The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p...
2022-08-25_Effect of Cu and chelators on metaciclogenesis.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 1.3 KB - 4 Variables, 63 Observations - UNF:6:NA2hRqSDBRp29MQtOjThIA== Quantification of metacyclogenesis: Epimastigotes were maintained in mid-log phase by dilutions every 2-3 days in LIT-10 % FBS 5 μM hemin medium, at least for 10 days. Then, the cells were washed with PBS, diluted at a concentration of 5 x 106/mL and incubated in LIT-10 % FBS 5 μ...
2022-09-09_Growth curves 3_BCS and NeoCup treatment.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 2.3 KB - 20 Variables, 27 Observations - UNF:6:thArFcR7AcyCuj8Nu1eRsA== Growth curves_ General Method The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p...
2022-09-30_Oxygen consumption with Cu_BCS_NeoCup_day3.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 509 B - 3 Variables, 27 Observations - UNF:6:B+hxSkK8spZK+CkDCydxFA== 2022-09-30_Oxygen consumption with Cu_BCS_NeoCup_day3 Oxygen consumption was measured in epimastigotes grown in LIT-10 % FBS-5 μM hemin medium plus copper (Cu), BCS, NeoCup or no addition (control), for 3 days. Concentration in μM. The oxygen consumption rates from epimastigotes...
2022-10-17_Oxygen consumption with BCS_day 5.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 124 B - 3 Variables, 5 Observations - UNF:6:WrzC/jcJWRlO3nSkM+QT3Q== 2022-10-17_Oxygen consumption with BCS_day 5 Oxygen consumption was measured in epimastigotes grown in LIT-10 % FBS-5 μM hemin medium plus BCS or no addition (control), for 5 days. Concentration in μM. The oxygen consumption rates from epimastigotes were quantified from the line...
2022-11-08_Intracelular heme.7z Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Compressed Archive - 229.4 KB - MD5: bd336bb7e278bb9a0bee9f711c94b670 The file “2022-11-08_Intracelular heme/2022-11-08_Samples.xlsx” shows the sample list for Heme content determination. The spectrums are recorded in separate files named as "PVC WAVE-SCAN 2022-11-08 ..." (22 files). Method: Intracellular heme content determined by pyridine method...
2022-11-08_ROS test_in triplicates_3rd.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 94 B - 14 Variables, 3 Observations - UNF:6:UU9CR9M0aw9FG4gnMhbaZQ== Superoxide anion production in epimastigotes Superoxide anion production was quantified as it was previously described (https://doi.org/10.1016/j.cryobiol.2013.05.005) with some modifications. Epimastigotes were maintained for 10 days in mid-log growth phase by periodic dilutions...
2023-06_ICP.tab Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi Tabular Data - 88.0 KB - 21 Variables, 578 Observations - UNF:6:ZEtNF1KCjgywO7+wvOVbUA== ICP-OES Cu, Fe, Ca, K, Mg, Mn, Na, P, S, and Zn contents were determined by ICP-OES as described in (https://doi.org/10.7554/eLife.64690). Briefly, epimastigotes were maintained for 6 days in LIT-10 % FBS-5 μM hemin medium plus different concentrations of copper or copper chelat...

2022-01-17_Effect of Cu and chelators in the infection_3rd.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 48.6 KB - 48 Variables, 508 Observations -

Infection assay: Vero cells (ATCC CCL-81) were seeded on 12 mm round coverslips in 24-well culture plates, at a density of 1.58 x 103 cells/cm2, and incubated in DMEM- 2 % FBS. After 24 h, they were infected with cell-derived trypomastigotes (MOI of 10) for 16 h in DMEM-2 % FBS s...

2022-02-02_Growth curves 1_ Starved cells plus Cu 50 a 250.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 1.5 KB - 18 Variables, 20 Observations -

Growth curves_ General Method The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® p...

2022-02-11_Growth curves 2_Copper trearment.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 1.8 KB - 20 Variables, 19 Observations -

2022-08-25_Effect of Cu and chelators on metaciclogenesis.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 1.3 KB - 4 Variables, 63 Observations -

Quantification of metacyclogenesis: Epimastigotes were maintained in mid-log phase by dilutions every 2-3 days in LIT-10 % FBS 5 μM hemin medium, at least for 10 days. Then, the cells were washed with PBS, diluted at a concentration of 5 x 106/mL and incubated in LIT-10 % FBS 5 μ...

2022-09-09_Growth curves 3_BCS and NeoCup treatment.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 2.3 KB - 20 Variables, 27 Observations -

2022-09-30_Oxygen consumption with Cu_BCS_NeoCup_day3.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 509 B - 3 Variables, 27 Observations -

2022-09-30_Oxygen consumption with Cu_BCS_NeoCup_day3 Oxygen consumption was measured in epimastigotes grown in LIT-10 % FBS-5 μM hemin medium plus copper (Cu), BCS, NeoCup or no addition (control), for 3 days. Concentration in μM. The oxygen consumption rates from epimastigotes...

2022-10-17_Oxygen consumption with BCS_day 5.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 124 B - 3 Variables, 5 Observations -

2022-10-17_Oxygen consumption with BCS_day 5 Oxygen consumption was measured in epimastigotes grown in LIT-10 % FBS-5 μM hemin medium plus BCS or no addition (control), for 5 days. Concentration in μM. The oxygen consumption rates from epimastigotes were quantified from the line...

2022-11-08_Intracelular heme.7z

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Compressed Archive - 229.4 KB -

The file “2022-11-08_Intracelular heme/2022-11-08_Samples.xlsx” shows the sample list for Heme content determination. The spectrums are recorded in separate files named as "PVC WAVE-SCAN 2022-11-08 ..." (22 files). Method: Intracellular heme content determined by pyridine method...

2022-11-08_ROS test_in triplicates_3rd.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 94 B - 14 Variables, 3 Observations -

Superoxide anion production in epimastigotes Superoxide anion production was quantified as it was previously described (https://doi.org/10.1016/j.cryobiol.2013.05.005) with some modifications. Epimastigotes were maintained for 10 days in mid-log growth phase by periodic dilutions...

2023-06_ICP.tab

Oct 23, 2024 - Raw data about control of copper ion homeostasis in Trypanosoma cruzi

Tabular Data - 88.0 KB - 21 Variables, 578 Observations -

ICP-OES Cu, Fe, Ca, K, Mg, Mn, Na, P, S, and Zn contents were determined by ICP-OES as described in (https://doi.org/10.7554/eLife.64690). Briefly, epimastigotes were maintained for 6 days in LIT-10 % FBS-5 μM hemin medium plus different concentrations of copper or copper chelat...

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