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161 to 170 of 185 Results

Ballari_et_al_Supporting_Information_03_Compounds_Characterization.pdf Mar 5, 2024 - Supplementary data for: Amino Acids metabolism as drug target for anti-trypanosomatids drug discovery Adobe PDF - 3.1 MB - MD5: af9f6713537b5c3bb1a9edf1aa659f7c Compounds characterization. UV-vis characterization of the fluorescent proline analogue 1-NBD, as well as NMR spectral characterization of all final compounds. Prediction of physicochemical properties of fluorescent analogues was performed using molinspiration platform (http://...
Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Oct 2, 2023 Bortolotti, Ana; Palavecino, Alejandro; Sartorio, Mariana Gabriela; Carrillo, Néstor; Cortez, Néstor, 2023, "Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3", https://doi.org/10.57715/UNR/HETWJ9, RDA UNR, V1, UNF:6:8q3ySl5zvU6gu629Yxe3pQ== [fileUNF] Este conjunto de datos muestra la participación en la defensa antioxidante, de las dos ferredoxina/flavodoxina NADPH oxidorreductasas de bacterias FPRs y del transportador de electrones flavodoxinaver3 fldver3. Los mismos fueron recolectados tras realizar una caracterización ciné...
01_FPRs_Fld_y_Fds_Ver3_aminoacid_sequences.txt Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Texto sin formato - 1.2 KB - MD5: 4cba3480ca01d60319b6c33481466e6c Aminoacid sequences under study from Acinetobacter Ver3
02_FPR1_diferential_spectra.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 476.9 KB - 64 Variables, 1362 Observations - UNF:6:3g/0qbdlPBYhkd0QTSBpSA== FPR1 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.
03_FPR2_diferential_spectra.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 465.8 KB - 62 Variables, 1353 Observations - UNF:6:C7vrAsVY94CO5Qy+HM2YcA== FPR2 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.
04_Fluorescence_Spectra_FPR1_and_FPR2.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 18.2 KB - 22 Variables, 107 Observations - UNF:6:8/rcNpTvNQxDWdbYfWA6Sg== Fluorescence intensities of FPR1 and FPR2. Excitation spectra between (400-500) nm, fixed emission wavelength 530 nm. Emission spectra between (490-600) nm, fixed excitation wavelength 450 nm. NADP concentration between ( 0-1) mM.
05_Activity_data.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 2.2 KB - 13 Variables, 35 Observations - UNF:6:mNvmz5Lr9CQXD7iqyD5jJw== Kinetic measurements of FPR1 and FPR2. Diaphorase activity with DCPIP and ferricianure as substrate. Cytochromo c reductase with FlavodoxinVer3 as substrate.
06_Native_gel_diaphorase.jpg Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Imagen JPEG - 750.4 KB - MD5: c42ab0263ae51bc07dc819d8f51cfb43 Lanes: Soluble extracts from Ver3 cells corresponding to 20 μg (1-4) and 30 μg (7-10) of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively. (5) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3.
07_Native_gel_Coomasie.jpg Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Imagen JPEG - 444.5 KB - MD5: 99aba76e9c0ccc708601efebcbc72ee2 Lanes: (7) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3, (5-2) soluble extracts from Ver3 cells corresponding to 20 μg of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively.
08_FPR1_western.jpg Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Imagen JPEG - 96.7 KB - MD5: a53e7b1a1c345628ec6c03beaae9cea6 Lanes: Inmunostaining of total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12) anti-FPR1ver3.

Ballari_et_al_Supporting_Information_03_Compounds_Characterization.pdf

Mar 5, 2024 - Supplementary data for: Amino Acids metabolism as drug target for anti-trypanosomatids drug discovery

Adobe PDF - 3.1 MB -

Compounds characterization. UV-vis characterization of the fluorescent proline analogue 1-NBD, as well as NMR spectral characterization of all final compounds. Prediction of physicochemical properties of fluorescent analogues was performed using molinspiration platform (http://...

Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Oct 2, 2023

Bortolotti, Ana; Palavecino, Alejandro; Sartorio, Mariana Gabriela; Carrillo, Néstor; Cortez, Néstor, 2023, "Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3", https://doi.org/10.57715/UNR/HETWJ9, RDA UNR, V1, UNF:6:8q3ySl5zvU6gu629Yxe3pQ== [fileUNF]

Este conjunto de datos muestra la participación en la defensa antioxidante, de las dos ferredoxina/flavodoxina NADPH oxidorreductasas de bacterias FPRs y del transportador de electrones flavodoxinaver3 fldver3. Los mismos fueron recolectados tras realizar una caracterización ciné...

01_FPRs_Fld_y_Fds_Ver3_aminoacid_sequences.txt

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Texto sin formato - 1.2 KB -

Aminoacid sequences under study from Acinetobacter Ver3

02_FPR1_diferential_spectra.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 476.9 KB - 64 Variables, 1362 Observations -

FPR1 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.

03_FPR2_diferential_spectra.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 465.8 KB - 62 Variables, 1353 Observations -

FPR2 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.

04_Fluorescence_Spectra_FPR1_and_FPR2.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 18.2 KB - 22 Variables, 107 Observations -

Fluorescence intensities of FPR1 and FPR2. Excitation spectra between (400-500) nm, fixed emission wavelength 530 nm. Emission spectra between (490-600) nm, fixed excitation wavelength 450 nm. NADP concentration between ( 0-1) mM.

05_Activity_data.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 2.2 KB - 13 Variables, 35 Observations -

Kinetic measurements of FPR1 and FPR2. Diaphorase activity with DCPIP and ferricianure as substrate. Cytochromo c reductase with FlavodoxinVer3 as substrate.

06_Native_gel_diaphorase.jpg

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Imagen JPEG - 750.4 KB -

Lanes: Soluble extracts from Ver3 cells corresponding to 20 μg (1-4) and 30 μg (7-10) of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively. (5) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3.

07_Native_gel_Coomasie.jpg

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Imagen JPEG - 444.5 KB -

Lanes: (7) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3, (5-2) soluble extracts from Ver3 cells corresponding to 20 μg of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively.

08_FPR1_western.jpg

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Imagen JPEG - 96.7 KB -

Lanes: Inmunostaining of total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12) anti-FPR1ver3.

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