Facultad de Ciencias Bioquímicas y Farmacéuticas

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81 to 90 of 121 Results

10_DESK_DESR_H335Q_complex model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 27.1 MB - MD5: 8887b43113362d30efe65835ec76ad2a Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server. The DesK His188 was mutated to glutamine in silico
1_Sequence_alignment_of_DesK.tab Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases Tabular Data - 229.8 KB - 224 Variables, 306 Observations - UNF:6:JChUDRZ6/doYy9jW2t+zgQ== Homologs of DesK in different organisms. Sequence alignment get from a BLASTP using DesKC as query and excluding other Bacilus from the data base by CLUSTALW
2_DesK_wt_and_variant-activities_Bgal.tab Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases Tabular Data - 458 B - 10 Variables, 10 Observations - UNF:6:+4DfY4QqrGJfiBAbXHTnww== Signal translation in DesK and its variants. Bacillus subtilis ∆desk cells expressing either DesK WT and its variants H335A, H335Y, H188E, H188Q, H335Q or H188Q/H335Q were grown at 37 °C to an OD550 of 0.3, and then divided in two flasks. One was maintained at 37 °C (red bars), a...
3_Western_blot-DesK_wt_and_variant.jpeg Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases JPEG Image - 36.0 KB - MD5: 7241e1860d16485d148aecf1c85b091b Expression and membrane integration levels of DesK variants. Western blots of the membrane fraction of Bacillus cells expressing each DesK variant (DesK WT, H335A, H335Y, H335Q, H188Q/H335Q, H188E and H188Q).
4_Autophosphorylation_curve_DesK_wt_and_variant.tab Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases Tabular Data - 435 B - 3 Variables, 24 Observations - UNF:6:AChpgPX6NtVSFF17J9LNVg== Phosphorylation kinetics of DesKC WT and H335Q. Eight samples taken in the initial 30 seconds were analyzed with radioactive ATP
5_EnvZ_wt_and_variant_activities_Bgal.tab Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases Tabular Data - 3.7 KB - 24 Variables, 27 Observations - UNF:6:ZPUlJAEn/sRUWeYCsfZZZQ== β-galactosidase activity from a PompC-lacZ reporter fusion was measured at low osmolarity (Sucrose 0%) or high osmolarity (Sucrose 15%)
6_DESK_HIS_protein model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 12.7 MB - MD5: a640b7c9c7edebabcbdc6af3497c9dfb DesK structure in the phosphotransfer state (PDB 7JJI), where the H188E mutation was reverted to its WT.
7_DESK_GLU_protein model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 14.0 MB - MD5: 26e5011338644080dc253017b9d892e2 DesK structure in the phosphotransfer state (PDB 7JJI), where the H188E mutation was modified to H188Q.
8_DESK_DESR_WT_complex model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 23.0 MB - MD5: 52c3017fbd2667898741e8de386229f6 Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server.
9_DESK_DESR_H335Y_complex model.pdb Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases application/vnd.palm - 27.1 MB - MD5: 882c896df053c365b150957752e0cb99 Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server. The DesK His188 was mutated to tyrosine in silico

10_DESK_DESR_H335Q_complex model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 27.1 MB -

Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server. The DesK His188 was mutated to glutamine in silico

1_Sequence_alignment_of_DesK.tab

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

Tabular Data - 229.8 KB - 224 Variables, 306 Observations -

Homologs of DesK in different organisms. Sequence alignment get from a BLASTP using DesKC as query and excluding other Bacilus from the data base by CLUSTALW

2_DesK_wt_and_variant-activities_Bgal.tab

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

Tabular Data - 458 B - 10 Variables, 10 Observations -

Signal translation in DesK and its variants. Bacillus subtilis ∆desk cells expressing either DesK WT and its variants H335A, H335Y, H188E, H188Q, H335Q or H188Q/H335Q were grown at 37 °C to an OD550 of 0.3, and then divided in two flasks. One was maintained at 37 °C (red bars), a...

3_Western_blot-DesK_wt_and_variant.jpeg

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

JPEG Image - 36.0 KB -

Expression and membrane integration levels of DesK variants. Western blots of the membrane fraction of Bacillus cells expressing each DesK variant (DesK WT, H335A, H335Y, H335Q, H188Q/H335Q, H188E and H188Q).

4_Autophosphorylation_curve_DesK_wt_and_variant.tab

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

Tabular Data - 435 B - 3 Variables, 24 Observations -

Phosphorylation kinetics of DesKC WT and H335Q. Eight samples taken in the initial 30 seconds were analyzed with radioactive ATP

5_EnvZ_wt_and_variant_activities_Bgal.tab

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

Tabular Data - 3.7 KB - 24 Variables, 27 Observations -

β-galactosidase activity from a PompC-lacZ reporter fusion was measured at low osmolarity (Sucrose 0%) or high osmolarity (Sucrose 15%)

6_DESK_HIS_protein model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 12.7 MB -

DesK structure in the phosphotransfer state (PDB 7JJI), where the H188E mutation was reverted to its WT.

7_DESK_GLU_protein model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 14.0 MB -

DesK structure in the phosphotransfer state (PDB 7JJI), where the H188E mutation was modified to H188Q.

8_DESK_DESR_WT_complex model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 23.0 MB -

Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server.

9_DESK_DESR_H335Y_complex model.pdb

Jul 1, 2024 - Experimental evidence of a new mechanism of activation of bacterial histidine kinases

application/vnd.palm - 27.1 MB -

Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server. The DesK His188 was mutated to tyrosine in silico

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