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RAR Archive - 23.3 MB - MD5: 88f0c03eb4aba5e973bb26c862e63d4d
Inside this file can be found the raw data of all NMR experiments performed for the compound “Diol side product”, organized in folders with the following codes: NMR_1D_1H for the 1H NMR experiment; NMR_1D_13C for the 13C NMR experiment; NMR_2D_COSY for the 2D NMR experiment, COSY...
RAR Archive - 22.2 MB - MD5: 5e104ee65e2031c1b79c22a110f15ff9
Inside this file can be found the raw data of all NMR experiments performed for the compound “Diol monoacetate side product”, organized in folders with the following codes: NMR_1D_1H for the 1H NMR experiment; NMR_1D_13C for the 13C NMR experiment; NMR_2D_COSY for the 2D NMR expe...
RAR Archive - 24.4 MB - MD5: 1f7a78cfe037029edef2f0a7c71a6e47
Inside this file can be found the raw data of all NMR experiments performed for the compound “Diol monotriflate side product”, organized in folders with the following codes: NMR_1D_1H for the 1H NMR experiment; NMR_1D_13C for the 13C NMR experiment; NMR_2D_COSY for the 2D NMR exp...
TIFF Image - 154.9 KB - MD5: 5b24f6bcd2a0873a17eba017bc3f6a0a
Inside this image file can be observed the chemical structures of six compounds alkaloid type pyranoquinolone, studied in this dataset: Huajiaosimuline; Epoxyzantosimuline; Simulansine; Diol Side Product; Diol Side Product monoacetate; and Diol Side Product monotriflate.
application/vnd.palm - 27.1 MB - MD5: 8887b43113362d30efe65835ec76ad2a
Full-length DesK-DesR complex performed by protein-protein docking, using HDOCK server. The DesK His188 was mutated to glutamine in silico
Tabular Data - 229.8 KB - 224 Variables, 306 Observations - UNF:6:JChUDRZ6/doYy9jW2t+zgQ==
Homologs of DesK in different organisms. Sequence alignment get from a BLASTP using DesKC as query and excluding other Bacilus from the data base by CLUSTALW
Tabular Data - 458 B - 10 Variables, 10 Observations - UNF:6:+4DfY4QqrGJfiBAbXHTnww==
Signal translation in DesK and its variants. Bacillus subtilis ∆desk cells expressing either DesK WT and its variants H335A, H335Y, H188E, H188Q, H335Q or H188Q/H335Q were grown at 37 °C to an OD550 of 0.3, and then divided in two flasks. One was maintained at 37 °C (red bars), a...
JPEG Image - 36.0 KB - MD5: 7241e1860d16485d148aecf1c85b091b
Expression and membrane integration levels of DesK variants. Western blots of the membrane fraction of Bacillus cells expressing each DesK variant (DesK WT, H335A, H335Y, H335Q, H188Q/H335Q, H188E and H188Q).
Tabular Data - 435 B - 3 Variables, 24 Observations - UNF:6:AChpgPX6NtVSFF17J9LNVg==
Phosphorylation kinetics of DesKC WT and H335Q. Eight samples taken in the initial 30 seconds were analyzed with radioactive ATP
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