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21 to 30 of 42 Results

02_FPR1_diferential_spectra.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 476.9 KB - 64 Variables, 1362 Observations - UNF:6:3g/0qbdlPBYhkd0QTSBpSA== FPR1 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.
03_FPR2_diferential_spectra.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 465.8 KB - 62 Variables, 1353 Observations - UNF:6:C7vrAsVY94CO5Qy+HM2YcA== FPR2 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.
04_Fluorescence_Spectra_FPR1_and_FPR2.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 18.2 KB - 22 Variables, 107 Observations - UNF:6:8/rcNpTvNQxDWdbYfWA6Sg== Fluorescence intensities of FPR1 and FPR2. Excitation spectra between (400-500) nm, fixed emission wavelength 530 nm. Emission spectra between (490-600) nm, fixed excitation wavelength 450 nm. NADP concentration between ( 0-1) mM.
05_Activity_data.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 2.2 KB - 13 Variables, 35 Observations - UNF:6:mNvmz5Lr9CQXD7iqyD5jJw== Kinetic measurements of FPR1 and FPR2. Diaphorase activity with DCPIP and ferricianure as substrate. Cytochromo c reductase with FlavodoxinVer3 as substrate.
06_Native_gel_diaphorase.jpg Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 JPEG Image - 750.4 KB - MD5: c42ab0263ae51bc07dc819d8f51cfb43 Lanes: Soluble extracts from Ver3 cells corresponding to 20 μg (1-4) and 30 μg (7-10) of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively. (5) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3.
07_Native_gel_Coomasie.jpg Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 JPEG Image - 444.5 KB - MD5: 99aba76e9c0ccc708601efebcbc72ee2 Lanes: (7) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3, (5-2) soluble extracts from Ver3 cells corresponding to 20 μg of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively.
08_FPR1_western.jpg Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 JPEG Image - 96.7 KB - MD5: a53e7b1a1c345628ec6c03beaae9cea6 Lanes: Inmunostaining of total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12) anti-FPR1ver3.
09_FPR2_western.jpg Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 JPEG Image - 74.2 KB - MD5: 34bb32ba4cf0ccc31585a107ec3d459c Lanes: Inmunostaining of total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12) anti-FPR2ver3.
10_Coomasie_western.jpg Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 JPEG Image - 172.0 KB - MD5: 688dcc901306d9debf4f21a64473b536 Lanes: SDS-PAGE total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12).
11_qPCR_FPR1_and_FPR2.tab Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3 Tabular Data - 1.9 KB - 11 Variables, 33 Observations - UNF:6:mUT2iTME4TWzN0fJHEVRAg== Real time PCR quantification. Threshold cycle for fpr1ver3 and fpr2ver3 transcripts in untreated Ver3 cells, or after challenges with either 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV. controls were the expression of recA and rpoB housekeeping genes.

02_FPR1_diferential_spectra.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 476.9 KB - 64 Variables, 1362 Observations -

FPR1 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.

03_FPR2_diferential_spectra.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 465.8 KB - 62 Variables, 1353 Observations -

FPR2 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.

04_Fluorescence_Spectra_FPR1_and_FPR2.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 18.2 KB - 22 Variables, 107 Observations -

Fluorescence intensities of FPR1 and FPR2. Excitation spectra between (400-500) nm, fixed emission wavelength 530 nm. Emission spectra between (490-600) nm, fixed excitation wavelength 450 nm. NADP concentration between ( 0-1) mM.

05_Activity_data.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 2.2 KB - 13 Variables, 35 Observations -

Kinetic measurements of FPR1 and FPR2. Diaphorase activity with DCPIP and ferricianure as substrate. Cytochromo c reductase with FlavodoxinVer3 as substrate.

06_Native_gel_diaphorase.jpg

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

JPEG Image - 750.4 KB -

Lanes: Soluble extracts from Ver3 cells corresponding to 20 μg (1-4) and 30 μg (7-10) of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively. (5) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3.

07_Native_gel_Coomasie.jpg

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

JPEG Image - 444.5 KB -

Lanes: (7) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3, (5-2) soluble extracts from Ver3 cells corresponding to 20 μg of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively.

08_FPR1_western.jpg

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

JPEG Image - 96.7 KB -

Lanes: Inmunostaining of total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12) anti-FPR1ver3.

09_FPR2_western.jpg

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

JPEG Image - 74.2 KB -

Lanes: Inmunostaining of total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12) anti-FPR2ver3.

10_Coomasie_western.jpg

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

JPEG Image - 172.0 KB -

Lanes: SDS-PAGE total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12).

11_qPCR_FPR1_and_FPR2.tab

Oct 2, 2023 - Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3

Tabular Data - 1.9 KB - 11 Variables, 33 Observations -

Real time PCR quantification. Threshold cycle for fpr1ver3 and fpr2ver3 transcripts in untreated Ver3 cells, or after challenges with either 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV. controls were the expression of recA and rpoB housekeeping genes.

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