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Oct 2, 2023
Bortolotti, Ana; Palavecino, Alejandro; Sartorio, Mariana Gabriela; Carrillo, Néstor; Cortez, Néstor, 2023, "Datos espectroscópicos, cinéticos y fisiológicos de las dos Ferredoxin/flavodoxin-NADP(H) reductasas del aislamiento extremófilo Acinetobacter Ver3", https://doi.org/10.57715/UNR/HETWJ9, RDA UNR, V1, UNF:6:8q3ySl5zvU6gu629Yxe3pQ== [fileUNF]
Este conjunto de datos muestra la participación en la defensa antioxidante, de las dos ferredoxina/flavodoxina NADPH oxidorreductasas de bacterias FPRs y del transportador de electrones flavodoxinaver3 fldver3. Los mismos fueron recolectados tras realizar una caracterización ciné...
Tabular Data - 476.9 KB - 64 Variables, 1362 Observations - UNF:6:3g/0qbdlPBYhkd0QTSBpSA==
FPR1 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.
Tabular Data - 465.8 KB - 62 Variables, 1353 Observations - UNF:6:C7vrAsVY94CO5Qy+HM2YcA==
FPR2 absorbance data as a function of NADP concentration (0-1)mM. Wavelength range 300-600 nm.
Tabular Data - 18.2 KB - 22 Variables, 107 Observations - UNF:6:8/rcNpTvNQxDWdbYfWA6Sg==
Fluorescence intensities of FPR1 and FPR2. Excitation spectra between (400-500) nm, fixed emission wavelength 530 nm. Emission spectra between (490-600) nm, fixed excitation wavelength 450 nm. NADP concentration between ( 0-1) mM.
Tabular Data - 2.2 KB - 13 Variables, 35 Observations - UNF:6:mNvmz5Lr9CQXD7iqyD5jJw==
Kinetic measurements of FPR1 and FPR2. Diaphorase activity with DCPIP and ferricianure as substrate. Cytochromo c reductase with FlavodoxinVer3 as substrate.
JPEG Image - 750.4 KB - MD5: c42ab0263ae51bc07dc819d8f51cfb43
Lanes: Soluble extracts from Ver3 cells corresponding to 20 μg (1-4) and 30 μg (7-10) of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively. (5) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3.
JPEG Image - 444.5 KB - MD5: 99aba76e9c0ccc708601efebcbc72ee2
Lanes: (7) 0.25 μg of purified FPR2ver3, (6) 0.65 μg of purified FPR1ver3, (5-2) soluble extracts from Ver3 cells corresponding to 20 μg of total soluble protein at DO600nm = 0.2, 0.4, 0.8 and 1.2, respectively.
JPEG Image - 96.7 KB - MD5: a53e7b1a1c345628ec6c03beaae9cea6
Lanes: Inmunostaining of total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12) anti-FPR1ver3.
JPEG Image - 74.2 KB - MD5: 34bb32ba4cf0ccc31585a107ec3d459c
Lanes: Inmunostaining of total protein extracts from untreated cells or challenged with 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV-B (5 μg; 1-4), (15 μg; 5-8), (30 μg; 9-12) anti-FPR2ver3.
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